Total internal reflection structured light super-resolution imaging mode

TIRF-SIM imaging of the hollow structure of Clathrin-mediated endocytic vesicles: The TIRF-SIM modality, with an imaging thickness of only 100–200 nm, offers an excellent signal-to-noise ratio, making it the optimal imaging modality for observing biological processes near the plasma membrane. In the image, the clathrin-mediated endocytic vesicles appear as solid dot-like structures under wide-field imaging, while the hollow vesicular structure can be clearly observed under Multi-SIM super-resolution imaging.

Grazing incidence structured light super-resolution imaging modes

GI-SIM employs a proprietary grazing incidence illumination mode, with an imaging depth of 1 µm matching the depth of field of high numerical aperture objectives, maximizing the information collection efficiency of 2-D super-resolution imaging, making it ideal for observing the dynamic interactions of various organelles within the cytoplasm.

The Single Slice-SIM modality is a super-resolution imaging modality targeting a single layer within cells, enabling imaging at deeper levels of the sample. It also possesses slice-slicing capabilities comparable to confocal microscopy, effectively removing background fluorescence. Therefore, it is suitable for imaging intranuclear cells, small model organisms, and tissue sections.

3D structured light super-resolution imaging modal

It can perform high-speed three-dimensional super-resolution imaging of the whole cell volume, with a longitudinal (Z) imaging speed of 2.5 µm per second and an axial resolution of 320 nm. It can complete whole-cell 3D-SIM super-resolution imaging in just 3 seconds, which is the industry-leading performance.

Nonlinear structured light super-resolution imaging modes